Cherry_Biotech_Liver_Anatomy

AIMS

→ Increase biopsies viability in long term culture
→ Imaging and biochemical quantification of drug effect
→ Interface blood vessel/hepatocyte
→ DILI assessment based on the biochemical marker and morphometric read-out

FEATURES

→ Temperature: 37°C
→ Gas: normoxia & hypoxia
→ Perfusion mode: pulse
→ Culture unit: 24 well multi-well & 2 custom microfluidic chip
Cherry_Biotech_liver_biopsy_on_chip

Cherry_Biotech_liver_biopsy_on_chip

RESULTS

→ Metabolic functions maintain over 7 days (CYP450, EROD…)
→ First morphometric criteria acquired: necrosis assessment (edges, center)
→ Biochemical markers and DILI assessment ongoing
Cherry_Biotech_CubiX

Cherry_Biotech_liver_biopsy_slice_culture_microfluidics_chip

PERSPECTIVE

→ Going to super-resolution to image eventual effect of drugs on endothelial nanopores structures (supposed target of toxicity)
→ Going above 7 days to mimic chronic exposure (several plasmatic peaks patterns..)

drapeau-europe

This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 766181, project “DeLIVER”.