Vascularized Immunocompetent Organoids on a standard multiwell plate


 

 

> VASCULARIZE IMMUNOCOMPETENT MODEL

The patented microfluidic multiwell technology allows to interconnect wells with circulating drugs and immunocompetent cells through endothelium

>MAINTAIN IN UNIFORM SIZE AND SHAPE UP TO 95% OF SPHEROIDS

Keep your spheroids alive (From 15 to 91 per well), with <6% variation of the spheroid size.

> MEDIUM PERFUSION WITHOUT DETACHMENT OVER WEEKS

The patented anchoring point technology keeps the spheroids in each 24 microwells during perfusion

> COMPATIBLE WITH HIGH RESOLUTION IMAGING

Organoid tracking and automated image acquisition. The whole system fit in your microscope

The microwell’s adhesive bottom surface 4DCell’s technology anchors uniform spheroids in a transparent hydrogel fully compatible with live imaging. The combine Cherry Biotech’s microfluidic perfusion technology, create a membrane free functional vascular interface on top of the Spheroids by applying a constant shear stress to differentiate the endothelium layer. These combined technologies, uniquely preserve your spheroids in individual well with culture media (supporting constant nutrient supply) over weeks and allow to image in real time the effect of your drug or immunocompetent therapies. These combined technologies allow in a unique way to observe up to 2000 spheroids in parallel during several weeks on a 24 wells plate.

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Readouts & Customization

-Imaging (fluorescence, confocal,…)
-Immuno-Histo-Chemistry & supernatant dosage (cytokines, metabolites,…)
-Sensors (Lactate, pH, O2, Glucose)
-Cytotoxicity (MTT, live/dead assay) & Bystander effect
-qPCR, RT-PCR

-pH of the organoids 
-Necrotic or non-necrotic organoids core
-Customized Extra Cellular Matrix (Collagen, Matrigel, commercial matrix)
-From 2 to 8 replicates
-Organs interconection

 

We Are Here For You

Our team of experts for in vitro experiments service

1.

Discuss with our experts to determine the best models

2.

Determine the set of readouts and experimental plan

3.

We perform the study with at least 3 independent experiments

4.

Full report with access to raw data and option to send samples