Applications

Discover the applications of Cubix

LONG TERM KIDNEY TISSUE CULTURE

AIMS

→ Human kidney culture for 7 days

→ Automatic perfusion at a flow rate

→ Live optical readouts acquisition (fluorescence)

FEATURES

→ Temperature : 37°C

→ Gas : normoxia, 5% CO2

→ Perfusion mode : pulse

→ Culture unit : 24 well multi well

 

RESULTS

→ Human kidney has been maintained over 7 days of culture

→ Live observation allows following glomerulus development

→ Histology preserved compared to the previous protocol

PERSPECTIVE

→ Endocrine disruptors and nephrotoxic compounds testing and characterization

→ Extended to 3 weeks of culture for long term/chronic effect

HUMAN BIOPSIES CULTURE

AIMS

→ Tumor(s) cells sequential exposure to compounds in different wells (drugs, antibodies, reagents)

→ Maintain automatic perfusion at a constant flow rate

→ Live optical readouts acquisition (fluorescence)

FEATURES

→ Temperature: 37°C

→ Gas: normoxia

→ Perfusion mode: pulse

→ Culture unit: 24 well multi-well

RESULTS

→ Different medium culture circuits in multiple well, following customizable patterns

→ Cell & biopsies cultures maintained for 1 week

→ Cell tracking (contrast or fluorescence)

 

PERSPECTIVE

→ Endocrine disruptors and nephrotoxic compounds testing and characterization

→ Extended to 3 weeks of culture for long term/chronic effect

SPHEROID HUMAN KIDNEY CANCER ON CHIP

AIMS

→ Characterization and quantification of antimigration and antiproliferative effects of several molecules, mimicking acute exposure.

→ Detection of specific biomarkers

FEATURES

→ Temperature: 37°C

→ Gas: normoxia 5% CO2

→ Perfusion mode : pulse & continuous

→ Culture unit: custom microfluidic chip

 

RESULTS

→ Spheroids viable during a 5 days experiment with perfusion of media

→ EC-50 of each molecule has been determined

→ Live detection and following of the biomarkers inside the chip

PERSPECTIVE

→ Mimicking chronic exposure

→ Multiplexing of chips to identify drug effect on other organs

Cherry_Biotech_skin_on_chip_3

FULLY DIFFERENTIATED SKIN ON CHIP

AIMS

→ Obtain a fully differentiated healthy skin equivalent

→ Build a fully differentiated Melanoma on a chip

→ Detect CTC in the flow stream

→ Maintain the system for several weeks

FEATURES

→ Temperature: 37°C

→ Gas: normoxia, air-liquid interface

→ Perfusion mode : pulse & continuous

→ Culture unit: 6 well multi-well & trans well

 

RESULTS

→ Implementation of the protocol to fully differentiated skin equivalent

→ Establishment of the melanoma on a chip: ongoing

→ Implementation of optical detection of tagged CTC in the flow stream (signal detection based on GFP)

 

PERSPECTIVE

→ Testing anti-migration drugs

Fibroblasts were grown and cultivated for 7 days
Cardiomyocytes derived from iPSCs were cultured for 10 days. Contracting cardiomyocytes were obtained after 7 days of culture
Cherry_Biotech_heart_on_chip_cell-culture-cardiomyocytes_4_days
Cardiomyocytes derived from iPSCs were cultured for 4 days

HEART ON A CHIP

AIMS

→ Improve IPSc derived cardiomyocytes differentiation

→ Establish a Duchenne’s disease model on a chip

→ Combine electrical stimulations with biochemicals and bio-inductive extracellular matrix

FEATURES

→ Temperature: 37°C

→ Gas: normoxia, 5% CO2

→ Perfusion mode: pulse

→ Culture unit: custom microfluidic chip

Cardiomyocytes derived from iPSCs were cultured for 4 days

Cardiomyocytes derived from iPSCs were cultured for 10 days. Contracting cardiomyocytes were obtained after 7 days of culture

RESULTS

→ Electrodes combination, biochemical and cell culture chamber in a tailored chip

→ Electrophysiological current detection to follow the differentiation stage

→ Myofilament immunofluorescence: sarcomere detection

PERSPECTIVE

→ Standardize & Industrialize the protocol

→ Duchenne’s disease mechanism deciphering and drug testing

LIVER BIOPSY ON A CHIP

AIMS

→ Increase biopsies viability in long term culture

→ Imaging and biochemical quantification of drug effect

→ Interface blood vessel/hepatocyte

→ DILI assessment based on the biochemical marker and morphometric read-out

FEATURES

→ Temperature: 37°C

→ Gas: normoxia & hypoxia

→ Perfusion mode: pulse

→ Culture unit: 24 well multi-well & 2 custom microfluidic chip

RESULTS

→ Metabolic functions maintain over 7 days (CYP450, EROD…)

→ First morphometric criteria acquired: necrosis assessment (edges, center)

→ Biochemical markers and DILI assessment ongoing

PERSPECTIVE

→ Going to super-resolution to image eventual effect of drugs on endothelial nanopores structures (supposed target of toxicity)

→ Going above 7 days to mimic chronic exposure (several plasmatic peaks patterns..)

Capture through microimaging

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