Applications
Discover the applications of Cubix
LONG TERM KIDNEY TISSUE CULTURE
AIMS
→ Human kidney culture for 7 days
→ Automatic perfusion at a flow rate
→ Live optical readouts acquisition (fluorescence)
FEATURES
→ Temperature : 37°C
→ Gas : normoxia, 5% CO2
→ Perfusion mode : pulse
→ Culture unit : 24 well multi well
RESULTS
→ Human kidney has been maintained over 7 days of culture
→ Live observation allows following glomerulus development
→ Histology preserved compared to the previous protocol
PERSPECTIVE
→ Endocrine disruptors and nephrotoxic compounds testing and characterization
→ Extended to 3 weeks of culture for long term/chronic effect
HUMAN BIOPSIES CULTURE
AIMS
→ Tumor(s) cells sequential exposure to compounds in different wells (drugs, antibodies, reagents)
→ Maintain automatic perfusion at a constant flow rate
→ Live optical readouts acquisition (fluorescence)
FEATURES
→ Temperature: 37°C
→ Gas: normoxia
→ Perfusion mode: pulse
→ Culture unit: 24 well multi-well
RESULTS
→ Different medium culture circuits in multiple well, following customizable patterns
→ Cell & biopsies cultures maintained for 1 week
→ Cell tracking (contrast or fluorescence)
PERSPECTIVE
→ Endocrine disruptors and nephrotoxic compounds testing and characterization
→ Extended to 3 weeks of culture for long term/chronic effect
SPHEROID HUMAN KIDNEY CANCER ON CHIP
AIMS
→ Characterization and quantification of antimigration and antiproliferative effects of several molecules, mimicking acute exposure.
→ Detection of specific biomarkers
FEATURES
→ Temperature: 37°C
→ Gas: normoxia 5% CO2
→ Perfusion mode : pulse & continuous
→ Culture unit: custom microfluidic chip
RESULTS
→ Spheroids viable during a 5 days experiment with perfusion of media
→ EC-50 of each molecule has been determined
→ Live detection and following of the biomarkers inside the chip
PERSPECTIVE
→ Mimicking chronic exposure
→ Multiplexing of chips to identify drug effect on other organs
FULLY DIFFERENTIATED SKIN ON CHIP
FEATURES
→ Temperature: 37°C
→ Gas: normoxia, air-liquid interface
→ Perfusion mode : pulse & continuous
→ Culture unit: 6 well multi-well & trans well
RESULTS
→Maintenance of a fully differentiated skin (including epidermis and endothelium) and melanoma equivalent
→Exposed skin and melanoma to circulating immune cells (natural killers)
→Recapitulation of different invasion phases of melanoma
→Detection of metastatic cells (Circulating Tumor Cells, CTCs)
→Using a standard multiwell (+/- transwell)
→Implementation of optical detection of tagged CTC in the flow stream (signal detection based on GFP)
PERSPECTIVE
→ Testing anti-migration drugs
HEART ON A CHIP
AIMS
→ Improve IPSc derived cardiomyocytes differentiation
→ Establish a Duchenne’s disease model on a chip
→ Combine electrical stimulations with biochemicals and bio-inductive extracellular matrix
FEATURES
→ Temperature: 37°C
→ Gas: normoxia, 5% CO2
→ Perfusion mode: pulse
→ Culture unit: custom microfluidic chip
Cardiomyocytes derived from iPSCs were cultured for 4 days
Cardiomyocytes derived from iPSCs were cultured for 10 days. Contracting cardiomyocytes were obtained after 7 days of culture
RESULTS
→ Electrodes combination, biochemical and cell culture chamber in a tailored chip
→ Electrophysiological current detection to follow the differentiation stage
→ Myofilament immunofluorescence: sarcomere detection
PERSPECTIVE
→ Standardize & Industrialize the protocol
→ Duchenne’s disease mechanism deciphering and drug testing
LIVER BIOPSY ON A CHIP
AIMS
→ Increase biopsies viability in long term culture
→ Imaging and biochemical quantification of drug effect
→ Interface blood vessel/hepatocyte
→ DILI assessment based on the biochemical marker and morphometric read-out
FEATURES
→ Temperature: 37°C
→ Gas: normoxia & hypoxia
→ Perfusion mode: pulse
→ Culture unit: 24 well multi-well & 2 custom microfluidic chip
RESULTS
→ Metabolic functions maintain over 7 days (CYP450, EROD…)
→ First morphometric criteria acquired: necrosis assessment (edges, center)
→ Biochemical markers and DILI assessment ongoing
PERSPECTIVE
→ Going to super-resolution to image eventual effect of drugs on endothelial nanopores structures (supposed target of toxicity)
→ Going above 7 days to mimic chronic exposure (several plasmatic peaks patterns..)
Capture through microimaging
Attach cubix to your existing equipment, no extra equipment required
